The project of “investigation of biological resources of agriculture in Yunnan province and its peripheral area”belongs to National Basic Task Program of the Ministry of Science and Technology. Yunnan and its peripheral area are the concentrative areas inhabited by minority nationality people where the biological diversity of agriculture is quite abundance. However, the vicissitude is happening that minority cultures and biological resources of agriculture are disappearing gradually along with economic development and infiltration of external cultures. In order to conserve and develop traditional culture and biological resource of agriculture in the minority area, forties counties and ten minority nationalities were investigated and all tasks were completed as the project requires. The authors introduced conservation and utilization on biological resources of agriculture of minority nationality in Yunnan province and its peripheral area, in which the aim was focused on providing basic information and scientific evidences for conservation and development of traditional culture of minority, and constituting the conservation policy of biological resources and scientific researches.