Abstract: Eighteen SSR primer pairs were used to amplify SSR fragments for 96 strawberry cultivars. The 18 SSR primers generated a total of 184 bands among which 172 (93.5％) bands were found to be polymorphic. The number of polymorphic band amplified per primer pair ranged from 6 to 19, with a average of 9.56. The value of locus polymorphism information content (PIC) ranged from 0.5726 to 0.8885, and averaged 0.8057. The genetic similarity revealed by NTSYS and the clustering results of UPGMA showed that these 96 strawberry cultivars from different origins could not separately clustered well. There was no significant correlation between the genetic relationships and the origins of the cultivars. The cluster of Chinese landraces was relatively concentrated, while the genetic relationships between Chinese landraces and the developed cultivars in China were relatively farther. The genetic variations of the euramerican cultivars and Janpan cultivars were more obvious than those of the Chinese landraces and the developed cultivars in China. Key Words: Strawberry ; Genetic relationship; SSR