The full-length cDNA of APX gene was cloned from ramie(Boehmeria nivea L.）through RT-PCR and RACE methods according to the APX gene sequence of ramie transcriptome, named as BnAPX1. The full length cDNA of BnAPX1 was 1 201 bp, which has an open read frame of 870 bp, encoding a protein of 289 amino acids. Bioinformatic analysis showed that BnAPX1 protein shares the characters of plant peroxidase family. Amino acids sequence analysis suggested that BnAPX1 protein is highly similar to other APX proteins and is possessed of a transmembrance domain at its C-terminal. Real-time PCR analysis showed that BnAPX1 mRNA is expressed in the organs in ramie root, middle stem, shoot tip, stem bark, and young leaf. And the expression of BnAPX1 was the highest in youn leaf. BnAPX1 was also found to be up-regulated by Cd, which suggested that it might play a role on Cd stress.