“赣鄱英才555工程”领军人才培养计划项目“樟树全基因组研究”
樟树是我国特有的珍贵用材和经济树种,它富含多糖多酚、萜类等次生代谢物质,是香精香料、油脂化工、医药等的重要原料树种。本研究采用高通量测序技术(Illumina HiseqTM 2000)首次测定了樟树基因组大小,并利用生物信息方法估计樟树杂合率、重复序列情况、GC含量等基因组信息,为全基因组测序策略的选择提供依据。主要结论如下:(1)樟树基因组大小粗略估计为760 Mb左右;(2)樟树基因组有较高的杂合和一定的重复,杂合率约为0.65%;(3)由于樟树杂合率较高,全基因组鸟枪法策略不适合该基因组测序分析,可尝试使用BAC-to-BAC策略或fosmid策略,有利于樟树基因组的序列拼接和组装。
Cinnamomum camphora (L.) Presl is a kind of precious wood species and the main no-timber trees of special local product in our country and possess abundant the secondary metabolism,such as polysaccharides, polyphenols, oleicacid and terpenoid. C. camphora is a metallic element used in many important industries. In this study, the genome size of C. camphora was determined by new-generation sequencing technologies (Illumina HiseqTM 2000). Hybridity percentage, repeats and GC_depth were also estimated by bioinformatics. The main results were showed as following: (1) The average genome size of C. camphora was about 760 Mb; (2) Hybridity percentage of C. camphora was about 0.65%; (3) Whole-genome shotgun sequencing was not used to C. camphora genome sequencing, and the BAC-to-BAC or fosmid could be used to it.
伍艳芳,肖复明,徐海宁,等.樟树全基因组调查[J].植物遗传资源学报,2014,15(1):150-153.
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