油菜3个栽培种发芽期耐盐性评价
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农业部油料作物生物学重点实验室开放课题(2012-1);江苏省农业科技自主创新资金(CX(12)2028);农业部948项目(2011-G23)


Evaluation for Salt Tolerance of Three Cultivated Species of Rapeseeds at Germination Stage
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The Open Project of Key Lab of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture(2012-1),Jiangsu Innovation Fund for Agriculture Technology(CX(12)2028); 948 Project of of Ministry of Agriculture(2011-G23)

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    摘要:

    对现有油菜种质资源进行耐盐性评价是培育耐盐油菜品种的基础性工作。本研究在实验室内分别采用3种盐(NaCl)浓度(86mmol/L, 170mmol/L,256mmol/L)对芥菜型油菜(B. juncea)、白菜型油菜(B.rapa)和甘蓝型油菜(B.napus)等3个栽培种的203份种质资源进行盐胁迫后考察发芽率,结果表明盐胁迫对3个栽培种的发芽率有不同程度抑制作用,白菜型油菜表现最好,甘蓝型油菜次之,芥菜型油菜最差。基于发芽率筛选获得3个栽培种共14份优异种质。进一步利用梯度浓度盐(NaCl)溶液对其胁迫后,发现各栽培种的发芽率均随着盐浓度的升高呈逐渐下降趋势,符合Boltzmann曲线,但白菜型油菜下降最为缓慢。通过考察在上述3种盐浓度下各栽培种品系的6个性状值(胚轴长、根长、总鲜重、地上部分鲜重、总叶绿素含量和电导率)并比较后发现,白菜型油菜发芽期耐盐性优于甘蓝型油菜和芥菜型油菜。对170mmol/L NaCl胁迫下14份种质各性状值的主成份分析表明,白菜型油菜Br2、甘蓝型油菜Bn1和Bn3为最耐盐种质,可用于耐盐油菜品种的培育。

    Abstract:

    Evaluation of the salt tolerance on existed rapeseeds germplasms is the primary work for breeding salt-tolerance varieties. 203 accessions of three cultivated species of rapeseeds including B. juncea, B.rapa, B.napus were stressed by three NaCl concentrations (86mmol/L, 170mmol/L and 256mmol/L) and the germination rate (GR) was checked at germination stage. The results showed the GRs of three species were all inhibited but with different degrees under salt stress and B.rapa performed best, which followed by B. napus and B. juncea sequentially. Fourteen lines with good GRs were then stressed by gradient NaCl concentrations and results indicated that the GRs of three species decreased as the Boltzmann curve with the increasing NaCl concentrations, and B.rapa did most slowly all the time. Six physiological characters including germination index, germination rate, germination potential, root length, hypocotyl length, plantlet fresh weight, up-ground fresh weight, total chlorophyll content and electrical conductivity of the fourteen lines under the stress of three NaCl concentrations mentioned above were detected. It approved that the general salt tolerance of B.rapa was better than B. juncea or B.napus. The Principle Component Analysis (PCA) of the trait values under 170mmol/L NaCl stress revealed Br2 of B.rapa and Bn1 and Bn3 of B.napus were the best salt-tolerant germplasms which could be used in the future breeding application.

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龙卫华,陈松,浦惠明,等.油菜3个栽培种发芽期耐盐性评价[J].植物遗传资源学报,2014,15(1):32-37.

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  • 收稿日期:2013-04-24
  • 最后修改日期:2013-09-18
  • 录用日期:2013-09-26
  • 在线发布日期: 2013-12-19
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