Applying SSR molecular marker technology to analysis genetic diversity for 60 potato cultivars which contained liangshan landraces, varieties bred by xichang college and varieties bred by other place. We had Screened out 16 effective SSR primers. 68 loci had been found using those markers in the test materials. There were 42 polymorphic loci which accounted for 61.76%. The average value of PIC was 0.4717. The genetic distance among all materials had been calculated by NTSYSpc2.0, the average genetic distance between 2.43 to 3.96. The test materials had been divided into 3 categories through MEGA4 analysis software and in the dendrogram two Liangshan landraces was first separated out. The Liangshan landraces and other materials had been classified into two groups by PoPGEN. Those results indicated definite difference between Liangshan landraces and other materials in the genetic basis. So the Liangshan landraces are useful resource for breeding. This study provides some partly reference for application of Liangshan landraces potatoes in the further breeding.