本研究利用ISSR分子标记技术对国家种质武汉水生蔬菜资源圃收集并保存的来自全国各地的72份芋种质资源进行遗传多样性检测。研究结果表明,13条ISSR引物在72份芋种质资源中共扩增出109条带,其中 85条带具有多态性,多态性比率为78.51%;72份芋种质资源间遗传相似系数变幅的范围为0.56-0.97,说明ISSR标记能够揭示芋资源之间较高的遗传多样性;在遗传系数为0.725处,72份芋种质资源被聚为三个大类若干亚类,为进一步研究芋种质资源分类、起源、保存和利用奠定了基础。
Accessions of taro,collected from different parts of China were subjected to ISSR(inter-simple sequence repeats) analyses to assess prevalence of genetic diversity. With 13 ISSR primers,109 bands were amplified in 72 accessions of taro, of 85 bands were polymorphism. High genetic diversity was revealed by ISSR markers as ranges of similarity coefficient values was 0.56-0.97, The accessions were grouped by subjecting the f similarity values to UPGMA based clusting. At 0.725 genetic similarity value, all the 72 accessions could be grouped in 3 big clusters and several subclusters. The result will be helpful in efficient classification, evolutionary, conservation and utilization of genetic diversity of taro.
董红霞,柯卫东,黄新芳,等.基于ISSR标记的中国芋种质资源遗传多样性分析[J].植物遗传资源学报,2014,15(2):286-291.
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