Diamondback moth is one of the most destructive pests of cruciferous vegetables and causes great economical lost annually. The shortage of resistant plant resources has hindered the insect-resistant vegetable breeding progress. In this study, the resistances to diamondback moth were analyzed among the 218 accessions, which belong to 42 crucifer species and were collected from 10 different countries by the national mid-term gene bank for vegetables. The resistance level distributed almost evenly from highly resistance to highly sensitive, except that the extremely resistance or sensitive resources are rare. Significant differences in resistance were found between genus, species and subspecies. Even in same species, the resistant levels of accessions were widely distributed. In general, the cultivated resources showed higher resistance compared to the wild species on average, but wild species contained more high-resistant accessions. The north Europe and several other areas are the places being rich in resistant resources. By the present study, a total of 19 resistant resources were screened out, including four extreme high-resistant accessions. These materials will be valuable and useful in future vegetable breeding and anti-insect gene discovery.