Persimmon resources are rich in Zhejiang province, but they are complicated between varieties or types. To investigate genetic diversity of Persimmon in Zhejiang, 40 Persimmon trees of 7 types were RAPD using 16 selected random primers. 156 fragments were showed in total, and in which 138 were Polymorphisms fragments. Average percentage of polymorphic loci was 87.76%, genetic diversity index (Dst) between types was 0.7604, outclass that within type (Hs) of 0.0603. The coefficient of differentiation (Gst) was 0.7604, and the gene flow was 0.1576. At last, 7 types of Persimmons were clustered according to the genetic distance between then. the result showed that genetic structure were basically identical within each type, but there were lot of different between 7 types, and some gene communication. Widely collection of Persimmons resources should be researched in future, so as to lay a foundation for the conservation and fine variety breeding of Persimmons