In 2011 and 2012, the resistance to sugarcane mosaic disease caused by Sorghum mosaic virus (SrMV-HH, GenBank accession number DQ530434) was identified and evaluated in 49 new improved varieties and 19 elite intermediate materials of Yunzhe series by dropping inoculation on stem-section and SrMV RT-PCR detection. The results showed that of the 49 new improved varieties, 29 (59.18%) were highly (Grade 1) to moderately resistant (Grade 3) to SrMV. Nine varieties were highly resistant (Grade 1) to SrMV, which included Yuegan 40, Yuegan 42, Yuetang 55, Yunzhe 03-194, Yunzhe 99-596, Yunrui 06-189, Guitang 30, Dezhe 03-83 and Mintang 01-77 and presented 18.37% of the 49 new improved varieties. Resistance (Grade 2) was found in 14.29% (7 out of 49) of the 49 new improved varieties, which included Funong 0335, Liucheng 05-129, Yuetang 96-86, Yunzhe 01-1413, Yunzhe 03-258, Yunzhe 06-80 and Guitang 02-351. Of the 19 fine intermediate materials of Yunzhe series, 13 (68.42%) were highly (Grade 1) to moderately resistant (Grade 3) to SrMV. In 19 elite intermediate materials of Yunzhe series, seven materials (Yunzhe 04-622, Yunzhe 05-197, Yunzhe 06-267, Yunzhe 05-194, Yunzhe 06-160, Yunzhe 07-2384 and Yunrui 05-704) were scored as highly resistant (Grade 1), counting for 36.84 % (7 out of 19). One material (5.26%), Yunzhe 06-362 were resistant (Grade 2) to SrMV. The results in the present study could provide scientific basis and valuable resistance resource for the furher breading of sugarcane cultivars against mosaic disease, and effective control of sugarcane mosaic disease by selecting and extending elite resistant cultivars.