In order to expand germplasm resources and rich genetic resources in rice breeding, 165 rice breeding lines introduced from International Rice Research Institute (IRRI) were evaluated for blast, bacterial blight and brown planthopper in 2011 and 2012 in Hubei Province, China. The resistance identification results showed that 19 lines were resistant to brown planthopper, 40 lines were resistant to bacterial blight, 14 lines were resistant to blast, 8 lines were resistant to both bacterial blight and brown planthopper, 7 lines were resistant to both bacterial blight and blast, and one lines were resistant to bacterial blight, brown planthopper and blast. Some of lines were used in breeding programme.