Abstract: The joint segregation analysis method of mixed major gene plus polygene genetic model was used to study the inheritance of CMV disease resistance in flue-cured tobacco．In two different environments, four generations (P1、P2、F1 and F2) from the crosses between Taiyan-8 which is high resistant to CMV disease as the maternal parent and NC82, susceptible to CMV disease, was used as the paternal parent were investigated. It was found that, Under the environment of green house, CMV resistance genetic model identified fit model E1 both at seeding stage and adult stage. That is CMV resistance genes were hybrid controlled by two additive-dominance-epistatic major genes and plus-dominant multiple genes, heritabilities of the major genes were estimated to be 37.11% and 57.76% respectively. Under the environment of field, the CMV disease resistance genetic model fit the additive-dominance-epistatic model (C0) and the heritability of the polygene was 26.86% at seeding stage. However, CMV resistance genetic model was identified to fit model E2 and heritability of the major genes was estimated to be 36.57% at adult stage. The results indicated that inheritance of Taiyan-8 resistance showed slightly difference in different environments and growth stages as the temporal and spatial expression of plant resistance genes. With the growth of tobacco, CMV resistant genes were controlled by two major genes and polygene in two different environments. So major-gene is a main factor in genetic improvement of CMV disease resistance whereas environmental effect should also be taken into consideration.