In this study, 555 Miscanthus sinensis germplasm resources were selected as material. Based on 26 phenotypic traits, 19 sampling strategies were used, which consisted of 3 stratification methods such as resource of geographical origin, floristic region and single character , 3 sampling number determination methods such as proportioned, square root and diversity index and 2 individual selection methods such as random and cluster. The criteria against whole collection included mean similarity coefficient, coincidence of traits, mean coefficient of variation of quantitaive traits and mean shannon index. The results indicated that an optimal strategy was set by stratification according to floristic region. determination of the sampling number with genetic diversity-dependent strategy and selecting sample by clusters. By using this strategy, a pre-core collection of Chinese Miscanthus sinensis germplam with a total of 83 accessions was established, carrying 100% phenotypic genetic diversity of the whole collection.