Glutelin is the most abundant storage protein in rice seeds, accounting for over 70% of the total seed protein, as well as the major rice protein absorbed. Rice with low glutelin content (LGC) is suitable for the special needs of patients suffering from diabetes and kidney failure. To improve the LGC rice variety, LGC-1, a LGC variety from Japan, was used to cross with “Wuyujing 3”, a japonica variety with high yield and quality in Jiangsu province. Three new LGC rice strains (F6 generation) with elite agronomic traits were obtained by agronomic traits selection and marker-assisted selection in 2013. SDS-PAGE analysis and glutelin content measurement showed that glutelin contents of the 3 new strains were reduced to LGC-1 level, significantly lower than ordinary commercial varieties.