As the research related to male sterility genes TA1 and TA2 expression characteristics on anther developmental stages of wheat thermo-sensitive male sterile line BNS and YS,to validate the relationship between these two sterility genes and fertility transformation of wheat thermo-sensitive male sterile line, mRNA expression level of TA1 and TA2 were examined by means of fluorescence Real-time quantitative PCR(qRT-PCR) taking the anthers of sterile line BNS and 732A as materials at four key developmental stages(tetrad phase,uninucleated period, binucleated period and trinucleate period).The results showed that:(1)The relative expression of TA1 increased from tetrad phase to binucleated period and decreased in trinucleate period of pollen abortion of sterile line BNS and 732A;(2)The relative expression of TA2 decreased from tetrad phase to binucleated period and increased in trinucleate period of pollen abortion of sterile line BNS,and the relative expression of TA2 increased from tetrad phase to binucleated period and decreased in trinucleate period of pollen abortion of sterile line YS;(3)The relative expression of TA1 and TA2 were extreme value in binucleated period of pollen abortion of sterile line BNS and YS,then the key stage of pollen abortion might be \"binucleated period\"for sterile line BNS and YS;(4)During a pollen development process of sterile lines BNS and 732,the variation range in relative expression of TA1 was higher than TA2. Then the influence degree of TA1 for pollen abortion sterile lines BNS and 732 might be better than TA2;(5)Gene TA1 and TA2 showed opposite effects on pollen abortion station of sterile line BNS,and were non-allelic genes in BNS genome. In pollen abortion station of sterile line 732A,the situation of them was just synergistic.