Auxin affects many processes of plant growth and development. As an auxin receptor, Auxin Binding Protein (ABP1) plays an important role in the rapid reactions induced by auxin on the plasma membrane. The ABP1 in common wheat, named TaABP1-D, have beenwas previously cloned, but not been located on theits chromosome map and the distribution in the cell are still not clear. The distribution of ABP1 in the cell is still unclear. In this study, we located the TaABP1-D on the cytoplasm and cell membrane by transiently expressed system in onion epidermal cells, which indicated that TaABP1-D protein is a membrane protein. In addition, using Chinese Spring nullitetrasomic-tetrasomic lines and bioinformatics methods, we located the TaABP1-D in the pericentromeric region of the long arm of wheat chromosome 5D. EST markers BE490079 and BE405060 flanked TaABP1-D by 0.51 and 0.28 cM, respectively.