福建省近年来审(认)定花生品种的遗传多样性分析
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泉州市科技重点项目2012N77


Genetic Diversity of Peanut Cultivars Registered in Fujian Province
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    摘要:

    以福建省近年来审(认)定的23个花生品种为材料,进行了包括株型、叶形、叶大小、叶色、果形、网纹粗细、网纹深浅、开花习性、粒大小、粒色和粒形共11个表型性状的Simpson遗传多样性指数(简称SI)和Shannon-Weaver遗传多样性指数(简称SWI)分析。结果表明:除开花习性、粒色和粒大小性状统一外,23个主栽品种的遗传多样性指数分别为SI=0.506,SWI=0.796,其中以果形最低(SI=0.237,SWI=0.387),以网纹粗细最高(SI=0.755,SWI=1.335);从50对引物中筛选出9条多态性较好引物,共扩增出59条多态性条带,平均每对引物可扩增6.56条多态性条带,引物的多态性信息含量 (PIC) 变幅为0.74~0.95,平均值为0.88。23个品种间的遗传相似系数在0.225~0.818,平均为0.579。在相似系数0.55处,可分为三个类群。系谱分析表明亲本来源相近的品种优先聚在一起,利用辐照诱变材料对于拓宽花生遗传基础发挥作用较明显。SSR分析结果基本上与系谱相吻合。

    Abstract:

    The study had analyzed the Shannon-Weaver and Simpson indexes of 11 phenotypic traits including plant type,leaf shape,leaf size,leaf color,peanut shell shape,thickness of skin texture,depth of skin texture,flowering habit,grain size,grain color,grain shape on 23 peanut varieties which were registered in Fujian Province.The results showed that flowering habit, grain color and grain size traits are all the same and the genetic diversity index of 23 peanut cultivars were SI = 0.506, SWI = 0.796 respectively, fruit shape was the lowest (SI = 0.237, SWI = 0.387) and textured thickness was the highest (SI = 0.755, SWI = 1.335). The genetic diversity among 23 peanut varieties was analyzed with 50 SSR markers, 9 SSR loci were polymorphism.59 bands were polymorphic, 6.56 polymorphic bands could be detected by each marker averagely, The polymorphism information content(PIC)was varied from 0.74 to 0.95,with an average of 0.88. The genetic similarity(GS) among 23 peanut varieties were calculated based on the data from these SSR markers, the value of GS ranged from 0.225 to 0.818,with an average of 0.579. UPGMA cluster analysis showed that the 23peanut varieties could be divided into three groups at similarities coefficient of 0.55. Most of the similar varieties were bred by the same breeders or belonged to the same type, which was in accordance with the pedigree information, the use of irradiation mutagenesis material for broadening the genetic base of peanuts obviously played an important role. The results of SSR analyzing genetic diversity are basically consistent with the pedigree.

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肖宇,陈剑洪,郭陞垚,等.福建省近年来审(认)定花生品种的遗传多样性分析[J].植物遗传资源学报,2015,16(4):728-733.

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  • 收稿日期:2014-07-07
  • 最后修改日期:2015-03-19
  • 录用日期:2015-05-27
  • 在线发布日期: 2015-06-11
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