The genetic diversity and structure of 6 natural populations in Hunan Province from 5 different regions was investigated using inter-simple sequence repeat(ISSR)markers.As a result,122 loci were identified from the 9 selected primers in 149 individuals of all populations,of which, 113 loci were polymorphic,The percentage of polymorphic bands (P) was high to 92.62%,while Nei’s gene diversity(H) and Shannon information index(I)were 0.3264 and 0.4873. The coefficient of gene differentiation (Gst)was0.5215,Gene flow among populations(Nm)was 0.4588, Analysis of molecular variance(AMOVA)demonstrated that the genetic differentiation among populations was relatively high. The among-population component accounts for 51.07%of the total variation, while the within-population component accounts for 49.83%. 6 populations were clustered into 3 groups by the UPGMA cluster analysis,the result showed that Da Wei Shan and Long Shan populations were in the first group,Yue Yang and DaoXian populations were in another group,and Heng Shan population separated from all the populations in the dendrogram, the geographical distribution was not related to the cluster results, but was mutually related to the geographical Elevation.