In order to create useful mutations and create new peanut germplasm, two different peanut lines were treated with five different concentration of EMS( 0 ,0.5％ , 1％, 1.5％, or 2％). Through injecting EMS into peanut flower organs. The agronomic characters and seed quality of M1 and M2 were analyzed. The results showed that the two lines mutagenic effect were obvious and the aberrations increased with the increasing concentration of EMS. The seed setting rate of the plants injected with EMS solutions and the seeding emergence rate of M1 generation are both lower than the control. Various types of mutated plants were discovered in M2 generations and most of the changed characters can be stably inherited. All these mutatied materials are conserved in germplasm library and will be in peanut functional genomic research and variety in provenment.