In this study, 16 male kiwifruit cultivars were analyzed using inter-simple sequence repeat markers (ISSR) to invettigate the genetic diversity. 10 primers selected from 100 primers were used for ISSR amplification. A total of 172 bands were generated, of which 140 bands were polymorphic bands(the percentage of polymorphic band, PPB=81.4%). The results of POPGENE version 1.32 analysis showed that the genetic distance of the 16 male kiwifruit cultivars was between 0.15-0.51 and the genetic diversity index (H) and Shannon index (I) was 0.24, 0.40, respectively. These cultivars were divided into 4 groups at the genetic similarity of 0.64. Class Ⅰ was made up of A. chinensis and A. deliciosa. Class Ⅱ was A. latifolia and A. eriantha. Class Ⅲ was 'Kuilv' and Class Ⅳ was A. tetramera. It is indicated that ISSR markers were feasible to analyze the genetic diversity of male kiwifruits and the results would facilitate the explotation and utilization of kiwifruits.