In order to provide base material on the conservation, research and development of stress-tolerant crop germplasm resources. Through referring literatures, visiting and field survey to investigate of stress-tolerant crop germplasm resources during 2011-2013 in arid region of Gansu province, including 81 villages in 48 towns of 18 counties. The survey collected local varieties and wild resources of grain crops, economic crops, vegetables, with the total 845 collected samples which belonged to 62 species of 46 genera in 16 families, including 27 drought-resistant germplasm, 4 salt-tolerant germplasm and 14 high resistant rust germplasm. In this study, local situation, growth, and decline conditions of crop germplasm resources were analyzed. Besides, the botanical classification and utilization importance of crop germplasm resources were estimated. Furthermore, the conservation, development, and utilization of crop germplasm resources in coastal areas of arid region of Gansu province were also discussed in the paper.