核桃品种绿岭杂交后代主要性状的遗传分析与优株选择研究
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十二五国家科技支撑计划项目(2013BAD14B03);河北省科技支撑项目(14236811D)


Study on the Main Traits Genetic Analysis and Superior Tree Selection of Hybrid Offsprings of ‘Lvling’ Walnut
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    摘要:

    为了培育出矮化、早实、丰产、优质等综合性状优于‘绿岭’的核桃新品种,以‘绿岭’核桃为亲本与‘辽宁1号’、‘绿早’进行杂交,测定了正反交组合374个杂交单株的13个性状,并对其进行了遗传分析,结果表明:杂交后代的表型性状具有较高的遗传多样性,各性状的变异系数均在17%以上,最高达70%;而果实相关性状除可溶性蛋白质之外,均在20%以下。青皮率和脂肪含量的遗传传递力在4个杂交组合中均大于97%,其遗传受加性效应影响较大。果实相关性状的广义遗传力均在90%以上,说明其在遗传上是比较稳定的,可以在早代进行选择。从杂交后代中初选21个优株,采用主成分分析的方法,对其进行评价和优株选择。结果表明,以性状的累积方差贡献率达到74.3%,确定了4个反映核桃主要性状的主成分;通过计算各单株的综合评价值,在供试的21个优株中,选出了3个综合性状优良的单株,其结果与单株的实际表型相近;并发现短结果枝率、矮化指数和节间长度3个指标能够反映出核桃的矮化优良性状。

    Abstract:

    In order to breed new walnut varieties with dwarf, precocious , fruitful and high quality traits which were superior were better than that of ‘Lvling’,, ‘Lvling’ walnut plants were used as parents crossed with ‘Liao ning NO 1’ and ‘Lvzao’ walnut, the 13 traits of 374 hybrid offspring in reciprocal crosses were investigated and itstheir genetic laws wasere analyzed. 13 traits of 374 strains in reciprocal crosses which ‘Lvling’ walnut was as a parent were investigated, and its genetic law was analyzed. The results showed that there existed relatively higher genetic diversities among the phenotypic traits of the hybrid offspring , the variation coefficients of each traits were more than 17%, the highest value reached 70%;%. But that of the fruit related traits were less than 20% except the soluble protein contents. In the 4 cross combinations, the inheritance of transfer ability of green husk percentage and kernel fat contents were more than 97%, that itsand their heredity was greatly influenced by additive effect. The broad-sense heritability of the fruit related traits were more than 90%, and this indicated that their heredities were relatively stable, you can and could be choosen in the early generation. 21 superior trees were primarily selected from the hybrid offspring , principal component analysis methods were used to evaluate them and select superior trees. The results showed that ,according to the cumulative variance contribution which reached 74.3%, 4 principal components which reflected the main economic characters were determined; by calculating the comprehensive evaluation value of each plant, 3 excellent trees from 21 superior trees were selected, and the evaluation results were similar to those of and thetheir actual phenotypes of pereach plants was similar, and found 3 indexes, that were the short bearing branch rate、, dwarf index and internode length canould reflect the excellent dwarf character of walnut.

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赵爽,齐国辉.核桃品种绿岭杂交后代主要性状的遗传分析与优株选择研究[J].植物遗传资源学报,2015,16(5):1004-1012.

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  • 收稿日期:2014-09-24
  • 最后修改日期:2015-04-22
  • 录用日期:2015-07-01
  • 在线发布日期: 2015-09-15
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