Identification of rice blast resistance genes in main rice cultivars and main parents help to understand the distribution of resistance genes among varieties, and provide a reference for breeding disease-resistant varieties and the makeup of varieties. In this study, 24 cultivars from Liaoning Province were used to study the distribution of resistance genes and functions of resistance alleles. Primers were designed according to the conservative region sequence of nine resistance genes to amplify the coding region of 24 cultivars. The amplification results from 24 cultivars were compared and suggested that the 24 cultivars from Liaoning Province did not carry the Pi21、Pi36、Pi37 gene or resistance alleles. However, Pid2, Pid3, Pita and Piks/Piks/Pikm/Pikp existed within the 24 varieties with different types and different mutation frequencies. Among these resistance genes, the resistance alleles Pid2 and Pid3 were detected in 24 cultivars. Pita alleles were detected in four cultivars and the newly-found base mutations did not affect resistance. The Piks/Piks/Pikm/Pikp sequence was not found in the 24 cultivars. After being inoculated with rice blast fungus with AVR-Pik, result showed that the alleles carried by varieties Liaojing454 and Shennong265 may serve resistance functions.