Abstract:SRAP and TRAP markers were employed to investigate the genetic diversity and relationships of 36 bermudagrass (Cynodon dactylon) accessions, including 34 wild bermudagrass germplasm resources of Hebei Province. Ten primer pairs from 238 primers pairs of SRAP and ten primer pairs from 85 primers pairs of TRAP were selected and used for the present study. Ten primers pairs of SRAP and TRAP produced a total of 186 and 161 bands, including 156 and 132 polymorphic bands, averaged 15.6 and 13.2 polymorphic bands per primer pair, and the percentages of polymorphic sites were 83.4% and 81.0% in average, respectively. Combined the both markers with cluster analysis, the genetic similarity among all accessions ranged from 0.519 to 0.983 and was 0.7 in average. When the GS is 0.68, the 36 accessions were classified into four distinct clusters. The results indicated there was substantial genetic diversity among the Cynodon dactylon germplasms in Hebei province, provide a scientific basis for germplasm resources conservation and breeding fine cultivars of bermudagrass.