Based on molecular marker-assisted selection, four major QTL genes derived from Wangshuibai chromosome 3B, 6B, 4B, and 5A resistant to wheat scab were transferred into six different susceptible genetic backgrounds to improve wheat disease-tolerance in the Huang-huai-hai area, their resistance and agronomic traits were evaluated subsequently at BC1F3 and BC1F4 progeny lines. Results obtained from field trail for two consecutive years showed that the average percentage of diseased spikelet and fuasrium diseased kernel of the lines carried four QTLs was 12.2% and 6.3% while those of the susceptible recipient parents up to 59.1% and 44.2%, respectively, indicating the wheat lines performed more significantly than their recipient parents in the disease tolerance, and that the plant height and spike length showed apparently negative correlation with percentage of diseased spikelet and fuasrium diseased kernel, but there was not significant difference between lines and recipient parents in flag leaf width, fertile spikelet number, grain number and hundred grain weight. The resistance to wheat scab would be enhanced after pyramiding major QTLs in susceptible germplasm, therefore, the lines with four QTLs could be applied further in wheat breeding , but further backcrossing or cross with other varieties could be helpful in breaking the close linkage of major QTLs to the adverse agronomic traits.