The product of marker linked to downy mildew resistance from cabbage could be amplified on Chinese cabbage-cabbage disomic alien addition line 1 (AC1d) and its parents Chinese cabbage and cabbage. And the product of marker linked to TuMV resistance from cabbage could be amplified on AC1d and cabbage, the corresponding product could not be amplified on Chinese cabbage. The values of plant length, plant width, head length, vitamin C and soluble protein contents during mature vegetative period in selfed progenies of AC1d showed a wide range of variation beyond corresponding values of parents Chinese cabbage and cabbage, which offered an opportunity to screen translocation lines that meet the breeding aims.