Abstract: The project of “investigation of biological resources of agriculture in Guizhou province” (No. 2012FY110200) belongs to National Basic Task Program of the Ministry of Science and Technology. Guizhou province is in the Yunnan-Guizhou plateau where the landform is complexity and the climate is diversity, and also is the concentrative inhabited areas with minority nationality people, which is described as a proverb that is ‘There are different weathers in ten miles, and different minority nationality at the same mountains’. Just because of these, the biological diversity of agriculture is quite abundance in Guizhou province. Nothing is changeless, however, the vicissitude is happening that minority cultures, life style and customs, and relevant biological resources of agriculture are disappearing gradually along with economic development and infiltration of external cultures. In order to conserve and develop traditional culture and biological resource of agriculture in the minority area, forty two counties were general survey, and of which twenty one counties were systematically investigated. The authors introduced conservation and utilization on biological resources of agriculture of minority nationality in Guizhou province, in which the aim was focused on providing basic information and scientific evidences for conservation and development of traditional culture of minority, and constituting the conservation policy of biological resources and scientific researches.