Anlong, located in southwest Guizhou, is one of the typical karst desertification areas where are featured by richness and diversity in bio-agricultural resources. It has great significance of conservation and development of these traditional resources. The systematic investigation on distinctive bio-agricultural resources was carried out for 9 villages distributing in 4 townships of Anlong in 2014 by ways of professional team, doucuments review, local symposium and field work. The result showed that 220 samples of these resources and their geographic ecology data were investigated, collected and systemized. In this paper, the current situation of utilization, the reasons for the growth and decline of collected these resources were analyzed. The suggestions on the utilization, conservation and development for these resources were proposed.