Aquaporins (AQPs) are the members of the major intrinsic proteins (MIPs) family with extraordinary ability to transport water, and play important roles in modulation of water relationships in plants. In this study, based on the genome database of Pyrus communis L. ‘Bartlett’ (http://www.rosaceae.org/species/pyrus/pyrus_communis/genome_v1.0), the AQPs gene family of Pyrus communis were identified and the sequences characteristic, phylogenetic trees, gene structure, GO function and expression profiles were analyzed. The phylogenetic tree was constructed using MEGA 6.0.5 software with neighbor-joining method. The structure of the genes were analyzed by GSDS 2.0 software, the motif was analyzed by MEME program, and GO analysis was carried by AgBase v2.00 software. Their expression patterns in different tissues were studied with semi RT-PCR. The results showed that there were 54 PcAQPs genes which containing AQP feature domains and conserved motifs were identified systematically in Pyrus communis. According to the gene structure, conserved domains and phylogeny relationship, the PcAQPs proteins were classified into 4 subfamilies (PIP, TIP, NIP and SIP) and the structure difference was larger between different subfamily genes, however, the genes with close relationship cluster had similar structure. GO analysis showed that the majority of PcAQPs gene had transporter activity, involved in substance transportation, stress response, growth, metabolism and other biological processes, but there were obvious differences in cellular components and biological processes between different subfamily members. Semi RT-PCR results showed that most of PcAQPs genes were expressed in roots, stems, leaves, and fruits, and the expression patterns of different genes and different subfamilies were different. These results laid foundation for cloning and functional analysis of PcAQPs gene in Pyrus communis.