Plant response to low temperature stress is a complex network process, involving numerous of transcription factors regulation. In this paper, we had found 108 full length cDNA sequences encoding bZIP factors in rapeseed from a transcriptome database bioinformatics analysis under low temperature stress. Aim at sequence compare, chromosome distribution and cluster analysis, these sequences can be differently density distributed on the 19 chromosomes, performing dynamic change in cold resistance process. We also found these transcription factors in A subtribe, C subtribe, I subtribe, G subtribe, S subtribe gathered the most, the subtribe B and D subtribe rarely gathered. Quantitative analysis of the four genes differences performance in all stress stages, we found that the genes regulation has the same trend with the sequencing analysis, not only proved the reliability of the sequencing data, but also proved the dynamic regulation of rapeseed bZIP transcription factors in response to low temperature stress.