To study the distribution of radiation sensitivity and expression patterns of TaKu70 and TaKu80 genes that are core factors in DNA repair process, 259 wheat mini core collection genotypes were irradiated by γ-rays with 0, 100, 150 and 250 Gy doses. Through germination test, the damage rate of seedling height showed 3 types of function relationships with the doses of γ-rays irradiation: logarithmic, linear and power function. Using HD50 as the measurement of radiation sensitivity, the 259 mini core collection genotypes could be divided into four categories: sensitive (contained 10 genotypes), medium sensitive (contained 96 genotypes), medium insensitive (contained 101 genotypes), and insensitive (contained 52 genotypes). There were more sensitive and medium sensitive genotypes in the logarithmic function relationship. And the expression levels of TaKu70 and TaKu80 genes were up-regulated by increasing of γ-rays doses compared with 0 Gy, but not affected significantly. There were more medium sensitive and medium insensitive genotypes in the linear function relationship. And the expression levels of TaKu70 and TaKu80 genes were gradually up-regulated by increasing of γ-rays doses compared with 0 Gy. There were more medium insensitive and insensitive genotypes in the power function relationship. And the expression levels of TaKu70 and TaKu80 were up-regulated compared with 0 Gy, but up-regulated firstly and then down-regulated with the increasing of γ-rays doses, and the general expression peaked at 150 Gy.