结球甘蓝根肿病菌鉴定和种质抗性评价
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农业部国家现代农业产业技术体系项目(CARS-25); 江苏省科技支撑项目(BE2013429);江苏省农业科技自主创新基金项目 [CX(13)2004]共同资助。


Identification of Cabbage(Brasisica oleracea. var. Capital L.) Club Root Pathogen (Plasmodiophora brassicae. Wor.)and Assessment of Germplasm
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Agricultural ministry National modern agriculture industry technology system project(CARS-25); Jiangsu science and technology support project(BE2013429);Jiangsu Agricultural science and technology innovation fund projects[CX(13)2004]

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    摘要:

    采集湖北省长阳县火烧坪乡根肿病重发区的病土和病根,通过病原菌形态和PCR鉴定,确定是芸薹根肿菌,然后利用欧洲ECD鉴别系统确定生理小种为ECD17/31/13,此病原菌致病力极强。采用田间苗期人工接种鉴定,与田间成株期自然诱发鉴定相结合,对88份甘蓝种质进行抗性评价和筛选,结果表明:苗期获得1份高抗,7份抗病,17份耐病材料;成株期获得4份高抗,4份抗病,15份耐病材料。两个时期88份材料群体抗性鉴定级别基本一致,93.18%材料成株期病指比苗期高。CR21在两个时期均为高抗,抗性最强,表现稳定;CR55在苗期发病最严重,病指达到76.19,成株期为74.10;CR54在成株期发病最严重,病指达到81.54,苗期为75.97,两个时期发病率均达到100%。根肿病菌的鉴定和致病力的确定,及甘蓝种质抗性评价为抗病品种选育和抗病机理的研究奠定基础。

    Abstract:

    Morphological identification and PCR confirmed that the Plasmodiophora brassicae is the pathogen caused cabbage clubroot. Using European ECD identification system, the?pathogen?was?further?identified?as a?physiological?strain coded as ECD17/31/13.?Field?trial?of?88?cabbage?germplasm?artificially?inoculated?by?ECD17/31/13?was?conducted?in village Huoshaoping(HShP),?Hubei?Province,?to?assess?their?resistance?nature?to?the?clubroot. After?evaluation,? their resistant phenotypes?were?leveled?as?high?resistance?(HR),?resistance?(R),?tolerance?(T) and susceptible(S).?The?results?showed?that:? among?the?88?cabbage?germplasm?engaged,?one?was?HR,?seven?R?and?seventeen?T?in?seedling?stage,?and?four?HR,?four?R,?15?T? in?plant?stage.?Germplasm?CR21?was?the?HR?material?which?performed?the?best?in?both?stages?stably.?Materials?CR55?and?CR54?were?found?as?the?most?susceptible.?The?disease?index?of?CR55?and?CR54?were?76.19?and?75.97?in?seedling?stage,?and?74.10 ?and?81.54?in?plant?stage?respectively,?when?the?incidence?rates?of?CR55?and?CR54?were?100%?both.?The?taxonomic?and?virulence?evaluation?on?the?pathogen?of?clubroot?disease,?and?screening?resistant?germplasm?in?cabbage?would?bring?clue?to?further?understand?the?phytopathological?mechanism?underlying?and?material?for?plant?breeding.

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王神云,吴强,王红,等.结球甘蓝根肿病菌鉴定和种质抗性评价[J].植物遗传资源学报,2016,17(6):1123-1132.

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  • 收稿日期:2016-01-25
  • 最后修改日期:2016-09-19
  • 录用日期:2016-06-20
  • 在线发布日期: 2016-11-07
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