Flavonoids, as one of the important functional components in loquat flower, play a significant role in anti-inflammation and relieving cough. In this study, the flower flavonoid contents were analyzed in 55 accessions of loquat germplasm from National Fruit Fuzhou Loquat Gene-pool. The result showed that, the flavonoid contents among the germplasm were significantly different, ranged from 0.44 % to 2.28 %. The highest content (Zhuonan No.1) was 5.18 times higher than the lowest one (Xiangzhong No.25). The average flavonoid level of loquat germplasm from Fujian province was significant difference with that from Zhejiang province (P＜0.01), as well as Guizhou province and Guangxi Zhuang autonomous region (P＜0.05). One loquat germplasm of ultra-high flavonoid content was screened out in this study, which is a promising material for further research. Meanwhile, the classification index for loquat flower flavonoid content level was establishedin, which provides a basis for further exploitation of loquat flower.