The test of distinctness, uniformity and stability (DUS) of new plant varieties was the foundation for new varieties protection. Traits Selection is key to DUS test. To establish beardless iris DUS testing, 43 traits were selected preliminarily. After removing length of leave and stigma with R-type cluster analysis, 41 traits with strong operability and distinct difference were finally determined; Four grouping traits were selected by Q-type cluster analysis; The classification and distribution of traits were analyzed to ensure the feasibility of the test guide, which improved the completion of DUS test guideline and based for test and authorization of varieties.