In 2015 and 2016, the two main pathogenic sugarcane streak mosaic virus (SCSMV-JP1, GenBank accession number JF488064) and Sorghum mosaic virus (SrMV-HH, GenBank accession number DQ530434) were used as inoculums, and the double resistance to SCSMV and SrMV was identified and evaluated in 41 elite sugarcane innovative germplasm and parent from the hybrid F1 progenies of saccharum officinarum “Ludashi”and Erianthus rockii “Yundian95-19”by dropping inoculation on stem-section and RT-PCR detection. The results showed that of 41 elite sugarcane innovative germplasm and parent, 23 (53.49%) were highly (Grade 1) to moderately resistant (Grade 3) and 20 (46.51%) were susceptible (Grade 4) to highly susceptible (Grade 5) to SCSMV; 31 (72.09%) were highly (Grade 1) to moderately resistant (Grade 3) and 12 (27.91%) were susceptible (Grade 4) to highly susceptible (Grade 5) to SrMV. Ten germplasm (23.26%) were highly resistant (Grade 1) to resistant (Grade 2) to SCSMV and SrMV, which included Yun09-603, Yun09-604, Yun09-607, Yun09-608, Yun09-619, Yun09-622, Yun09-633, Yun09-635, Yun09-656, Yundian95-19 of 43 elite sugarcane innovative germplasm. Of these, six germplasm (Yun09-604, Yun09-607, Yun09-619, Yun09-633, Yun09-656, Yundian95-19) were the highly resistant (Grade 1) to SCSMV and SrMV, accounting for 13.95%. The results in the present study have determined the resistance of 41 elite sugarcane innovative germplasm and parent to the two main pathogen of mosaic disease, screened the 10 germplasm resistant to SCSMV and SrMV, provide scientific basis and valuable resistance resource for the further breeding of sugarcane cultivars against mosaic disease.