Abstract: Virus disease is one of the major disease of radish (Raphanus sativus L.). Turnip mosaic virus (TuMV) which is the most important causative virus causes great economical loss in radish. The virus-resistant materials in radish breeding are indispensable currently. In this study, the resistance to TuMV was identified among 150 accessions of representative radish germplasm, coming from 125 cities of 25 provinces in China and other 3 countries. As a result, one immune materials among 23 resistant materials were screened out by combing phenotyping and ELISA detection. These materials would be useful for resistant gene mapping and new radish variety development in future.