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高粱子粒单宁含量和颜色QTL分析
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国家高粱产业技术体系(CARS-06-01-06)； 国家自然青年科学基金(31601627)；辽宁省科学技术计划项目(2014027018)

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    摘要:

    高粱在我国酿酒行业和农业结构调整中都有重要作用。高粱籽粒单宁含量和粒色是高粱两个重要农艺性状，但有关高粱籽粒单宁和粒色分子遗传研究还不是很多，也没有调控高粱粒色基因克隆的报道。本研究用白粒低单宁的BTx623与浅粉高单宁Rio两个品种为亲本，构建了含325个株系重组自交系基因定位群体，开展高粱籽粒单宁和粒色基因定位研究。筛选出118个SSR标记和8个INDEL亲本多态标记用于定位群体株系的基因型检测，结合亲本和定位群体籽粒单宁和粒色两年测定数据，采用QTL IciMapping4.1软件中的完备区间作图法，对高粱籽粒单宁和粒色两个性状进行QTL定位，共检测到3个与单宁含量相关和6个粒色相关QTL位点。与单宁含量相关的QTL qTan4-1即为Sb04g031730基因，另外检测到新的qTan1-1（txp11-txp279，1.29%）和qTan2-1（txp298-SB1292，1.28%）。控制粒色6个QTL中，qGC1-1（1.00%和12.03%）在txp11-txp279之间；qGC1-2（2.64%和16.96%）在txp43-txp11之间；qGC1-3（22.81%）在1d1A3-sam44127标记之间；qGC2-1（1.00%）在txp72-txp298之间；qGC2-2（1.19%）在txp298-SB1292之间；qGC6-1（15.08%）位于第6染色体txp57-sam43683之间。本研究为高粱籽粒单宁和粒色两个性状的精细定位、基因克隆和分子辅助选择研究奠定了基础。

    Abstract:

    Sorghum plays an important role in white wine industry and agriculture supply-side reform. Grain tannin and color are two important agronomic traits, but molecular genetic mechanisms of these two traits were not clear, and there were no cloned genes refered to grain color in sorghum to now. 325 recombinant inbred lines were constructed with BTx623 (white grian and low tannin) and Rio (pink grain and high tannin). Combination of two years phenotype data, 118 simple sequence repeat (SSRs) and 8 insertion-deletion (INDELs) markers, and QTL IciMapping4.1 were used in mapping with inclusive composite interval mapping (ICIM) method. 3 and 6 QTLs were mapped, which related to content of grain tannin and seed color, respectively. QTL qTan4-1 was controlled by a known gene Sb04g031730, and the other two QTLs, qTan1-1 (txp11 - txp279, 1.29%) and qTan2-1 (txp298 ~ SB1292, 1.28%) were new in content of grain tannin QTL analysis. In grain color analysis, qGC1-1 (1.00% and 12.03%), qGC1-2 (2.64% and 16.96%), qGC1-3 (22.81%), qGC2-1 (1.00%), qGC2-2 (1.19%) and qGC6-1 (15.08%) were mapped between txp11 - txp279, txp43 - txp11, 1d1A3- sam44127, txp72-txp298 and txp298-SB1292, txp57- sam43683 (chr6), repectively. Our study paves a way to fine mapping grain tannins and color, gene cloning and marker-assisted selection breeding.

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白春明,王春语,王平,等.高粱子粒单宁含量和颜色QTL分析[J].植物遗传资源学报,2017,18(5):860-866.

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  • 收稿日期:2017-02-27
  • 最后修改日期:2017-04-06
  • 录用日期:2017-04-07
  • 在线发布日期: 2017-09-14
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