Quantitative trait loci (QTL) analysis of protein content and oil content in Soybean is providing the theoretical basis for quality breeding of higher content of the protein and oil. There were 100 BC2 lines of backcross introgression lines from Zhonghuang 13 and Zhonghuang 20. SSR marker polymorphism of the population of BC2F5 was analysed. The protein content and oil content were measured by near infrared spectroscopy. A linkage map including 20 linkage groups with a total length of 948.01 cM and an average genetic distance of 8.78 cM with a total of 108 SSR markers was constructed. 5 QTLs related to protein content and 9 QTLs related to oil content were detected, including Satt445-Sat_303 for oil content detected in two years and Satt445-Sat_303 and Satt543-Satt574 for protein content and oil content detected in the same year, Sat_389-Satt590, Satt238-Satt388 and Satt685-Sat_381 for oil content.