西藏豌豆资源蛋白质含量及遗传多态性分析
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西藏农牧学院,西藏农牧学院,西藏农牧学院,西藏农牧学院

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国家自然基金(No.31560362),西藏自治区重点项目(No.XZXTCX-2016)。


Analysis of Protein Content and Genetic Polymorphism in Pea Germplasm in Tibet
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XiZang Agiricultural And Animal Husbandry College,XiZang Agiricultural And Animal Husbandry College,XiZang Agiricultural And Animal Husbandry College,XiZang Agiricultural And Animal Husbandry College

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    摘要:

    为掌握西藏高原豌豆种质资源遗传差异、亲缘关系及种子蛋白质含量和成分,扩大豌豆种质资源库,本研究搜集整理了来自西藏不同生态地理环境条件下的54份豌豆材料。通过测定种子总蛋白、水溶性和盐溶性蛋白含量,分析了地理生态因子(经度 、纬度 、海拔)与总蛋白含量间的关系。利用SDS-PAGE 对54份豌豆材料种子水溶性和盐溶性蛋白,进行了电泳和遗传多样性分析。研究结果表明,54份豌豆材料种子总蛋白含量17.58%~28.67%,其中水溶性蛋白占86.12~91.40%,盐溶性蛋占4.76~8.29%。西藏豌豆种子总蛋白含量高低与经度呈显著正相关,与纬度呈一定正相关,而与海拔有一定的负相关。54份材料种子水溶性和盐溶性SDS-PAGE分别检测出蛋白谱带 1588条和699条。水溶性蛋白分出迁移率不同的谱带类型43 种,基因多样性指数在0~0.5,其相对分子量在24.87~149.54Ku,呈现出24.71~50.41Ku低分子量区和56.34~88.08ku高分子量密集区。盐溶性蛋白分离出迁移率不同的谱带类型24条,其基因多样型指数0~0.5,相对分子量在24.85~91.24 Ku。按海拔高度将54份豌豆材料分为4个地理类群,各类群基因多样性指数分别为0.23、0.18、0.35和0.31,Shannon信息指数分别为0.33、0.41、0.52和.46。表明豌豆蛋白的变异与海拔高度有关,聚类分析将参试种质分为7 类,表明水溶性和盐溶性蛋白能在一定程度上反映种质间的亲缘关系,为西藏豌豆资源开发和优良亲本选配提供理论依据

    Abstract:

    Fifty-four landraces of field pea from different ecological and geographical environment conditions of Tibet were collected for this study. Total seed protein content, water soluble and salt soluble protein content of above genotypes of field pea were examined. While, the relationships between the protein contents (total seed protein content, water soluble and salt soluble protein content) of above genotypes and geographical factors (longitude, latitude and elevation) were analyzed. The results showed that the total protein content of 54 pea seed samples ranged from 17.58% to 28.67%, among which water soluble proteins accounted for 86.12 to 91.40%, and salt soluble proteins accounted for 4.76 to 8.29%. The total protein content of Tibet field pea seed samples significantly correlated with longitude, and correlated with latitude, but negatively correlated with altitude. 1588 water soluble and 699 salt soluble protein bands were detected by SDS-PAGE electrophoresis; among them, 43 band types of water soluble protein and 24 band types of salt soluble protein based on migration rate were identified with diversity index ranged from 0 to 0.5. The relative molecular weight of water soluble proteins ranged from 24.87Ku to 149.54Ku, showing concentrated areas of low molecular weight (24.71~50.41Ku) and high molecular weight (56.34-88.08ku). The relative molecular weight of salt soluble proteins ranged from 24.85Ku to 91.24 Ku. The 54 pea landraces is divided into 4 geographical groups according to the altitude information, with genetic diversity indices of 0.23, 0.18, 0.35 and 0.31, and Shannon information index of 0.33, 0.41, 0.52 and 0.46. The results showed that the pea protein variation related to altitude. Cluster analysis divided the 54 field pea landraces into 7 groups, indicating that water soluble and salt soluble

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侯维海,王建林,旦巴,等.西藏豌豆资源蛋白质含量及遗传多态性分析[J].植物遗传资源学报,2018,19(2):252-262.

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  • 收稿日期:2017-07-03
  • 最后修改日期:2017-07-16
  • 录用日期:2017-08-01
  • 在线发布日期: 2018-03-19
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