To establish an easy bioassay for evaluating the salt tolerance in potato, four typical genotypes with different life-habits were treated under different NaCl concentrations in MS medium to evaluate the optimum concentration by scoring plant height, total biomass, bud fresh weight and rooting rate. Fifty-two potato materials were stressed by optimum concentration (100 mmol/L). The salt tolerance was carried out by using membership function and cluster analysis. Out of that, two genotypes 'Longshu No. 5' and 'LZ111' were extremely salt tolerant, while 'Qingshu No.9', 'Longshu No.8', 'Zhongshu No.14' and '04P48-3' were extremely salt susceptible. Thus, this research provided an in intro method for screening salt tolerance accessions, and the identified salt-tolerance genotypes become valuable potential for potato breeding.