葡萄F-box基因VvSLY1的克隆与表达分析

doi:10.13430/j.cnki.jpgr.20180111001

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葡萄F-box基因VvSLY1的克隆与表达分析
DOI:
                        10.13430/j.cnki.jpgr.20180111001
                    
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作者单位:江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室，南京210014,江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室，南京210014,江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室，南京210014,江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室，南京210014,江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室，南京210014,江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室，南京210014
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基金项目:江苏省自然科学基金项目(BK20160587) ；江苏省农业科技自主创新资金项目(CX(16)1013)

Cloning and Expression analysis of F-box gene SLY1 in Vitis vinifera L.

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Institute of Pomology, Jiangsu Academy of Agricultural Sciences/ Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014,Institute of Pomology, Jiangsu Academy of Agricultural Sciences/ Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014,Institute of Pomology, Jiangsu Academy of Agricultural Sciences/ Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014,Institute of Pomology, Jiangsu Academy of Agricultural Sciences/ Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014,Institute of Pomology, Jiangsu Academy of Agricultural Sciences/ Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014,Institute of Pomology, Jiangsu Academy of Agricultural Sciences/ Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014

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摘要:

SLEEPY1(SLY1)属于F-box蛋白,是SCF复合体的主要组成原件之一,在赤霉素信号转导过程中发挥着重要的调节作用。本研究以‘夏黑’葡萄为试材,利用电子克隆和RT-PCR技术克隆获得一个F-box蛋白基因全长cDNA序列,命名为VvSLY1。该基因全长为1096 bp,包含1个555 bp的完整开放阅读框(ORF),编码184个氨基酸。序列比对和结构域分析结果表明,该VvSLY1包含F-box蛋白家族的LGG和LSL保守结构域。进化树分析结果显示,VvSLY1与胡杨亲缘关系最近。qRT-PCR分析结果表明,在果实快速膨大发育阶段,外源GA处理会促进该基因的上调表达。

Abstract:

SLEEPY1(SLY1), one of the main elements in SCF complex, belongs to F-box protein. It plays an important regulatory role in the signal transduction process of gibberellin. A full length of F-box gene, designated VvSLY1, was isolated from grapevine ‘Summer Black’ (Vitis vinifera L.) by electronic cloning and RT-PCR method. The gene was 1096 bp and contained a complete open reading frame of 555 bp that encoded 184 amino acids. Sequence alignment and motif analysis suggested that the VvSLY1 gene contained LGG and LSL, which were conserved domains of F-box protein. Phylogenetic tree analysis showed that the VvSLY1 protein had the closest genetic relationship with the Populus euphratica protein. Results of qRT-PCR revealed that the expression of VvSLY1 was up-regulated by gibberellin treatment in the fast expansion development of fruit.

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王西成,赵密珍,周蓓蓓,等.葡萄F-box基因VvSLY1的克隆与表达分析[J].植物遗传资源学报,2018,19(5):959-966.

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收稿日期:2018-01-11
最后修改日期:2018-04-14
录用日期:2018-05-14
在线发布日期: 2018-09-14
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