农户保存与种质库保存的同近名地方稻品种的遗传多样性研究
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湖南大学研究生院隆平分院,湖南省农业科学院水稻研究所,湖南省农业科学院水稻研究所,湖南省农业科学院水稻研究所,湖南省农业科学院水稻研究所,湖南省农业科学院水稻研究所,湖南省农业科学院水稻研究所,湖南省农业科学院水稻研究所/农业部长江中下游籼稻遗传育种重点实验室,湖南大学研究生院隆平分院,湖南大学研究生院隆平分院

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农业部物种资源保护费项目-第三次全国农作物种质资源普查与收集行动(1120162130135252036);国家现代农业产业技术体系建设专项(nycytx-001)第一作者金建楚,在读硕士生。研究方向水稻种质资源评价。Email:879878589@qq.com通讯作者李小湘,研究员。研究方向水稻种质资源评价。Email:xiaoxiang66196@126.com


SSR Markers and Phenotypic Analysis In-Situ Conservation in Local Farmers with Ex- Situ Conservation in Low-temperature Rice Resources
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Long Ping Branch,Graduate School of Hunan University,Hunan Institute of Rice Research,Hunan Academy of Agricultural Sciences,Hunan Institute of Rice Research,Hunan Academy of Agricultural Sciences,Hunan Institute of Rice Research,Hunan Academy of Agricultural Sciences,Hunan Institute of Rice Research,Hunan Academy of Agricultural Sciences,Hunan Institute of Rice Research,Hunan Academy of Agricultural Sciences,Hunan Institute of Rice Research,Hunan Academy of Agricultural Sciences,Hunan Institute of Rice Research,Hunan Academy of Agricultural Sciences,Long Ping Branch,Graduate School of Hunan University,Long Ping Branch,Graduate School of Hunan University

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    摘要:

    本研究通过对湖南农户自留种原地保存(简称农户保存)与异地低温保存在湖南种质库(简称低温保存)的同近名水稻资源间的表现型差异显著性以及SSR标记遗传相似性比较分析,7组( 同近名材料为6组) 共92份为研究材料,目的是鉴定同近名资源间遗传距离,为之后有效保存、针对性供种、高效利用提供理论依据。研究结果表明,供试同近名材料在表现型性状上差异有统计学意义,且农户保存与低温保存的同近名材料在表现型性状上差异有统计学意义。SSR标记比较农户保存与低温保存湖南同近名材料,发现除E组材料外,其它组材料都是农户保存的等位基因变异数小于低温保存,说明农户保存在年复一年的种植、收种过程中进行了自然和人工选择。在同近名组内SSR标记相似系数,除C组外,农户保存的材料间>低温保存的材料间>农户保存材料与低温保存材料间。说明农户自留种保存的同名水稻资源值得收集评价。

    Abstract:

    In this study, we analyzed the phenotypic differences between Hunan peasant households preserved in situ (hereinafter referred to as farmer households) and their hypothermic counterparts in Hunan germplasm pool (hereinafter referred to as cryogenics preservation), and the genetic similarity of SSR markers comparative analysis,A total of ninty two accessions of rice( Oryza sativa L.) landraces divided into 7 groups(the same name and similar names of the material for the 6 groups),the purpose is to identify the genetic distance with the resources of the same name, to provide a theoretical basis for effective conservation, targeted supply and efficient use.The results showed that there were significant differences in the phenotypic traits of the same name for the test materials, and there was significant difference in the phenotypic traits between the farmer"s preserved and the cryogenic preserved materials.Comparison of SSR markers between farmer and low-temperature preservation of Hunan with the same name material, found that in addition to the E group of materials, other groups of materials are farmer preservation of allelic variation is less than cold preservation.It shows that the farmers" preservation is natural and artificial selection in the process of planting and harvesting year after year.In the group with the same name of similar SSR markers similar coefficient, except for Group C, farmer conserved materials > materials stored in low temperature > farmer conserved materials and low temperature preservative materials.It is suggested that the farmers" retention of the same name rice resources is worth collecting and evaluating.

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金建楚,李小湘,黎用朝,等.农户保存与种质库保存的同近名地方稻品种的遗传多样性研究[J].植物遗传资源学报,2018,19(3):478-487.

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  • 收稿日期:2018-01-12
  • 最后修改日期:2018-03-14
  • 录用日期:2018-03-05
  • 在线发布日期: 2018-05-08
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