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首页 > 过刊浏览>2019年第20卷第1期 >48-59. DOI:10.13430/j.cnki.jpgr.20180612002
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新麦草种质的SSR遗传多样性及群体结构分析
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基金项目:

国家自然科学基金项目（31460627）；内蒙古农业大学科技成果转化专项（YZGC2017012）；


Genetic Diversity and Structure Analysis in Psathyrostachys Population Using SSR Markers
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Fund Project:

The Project of National Natural Science Foundation of China 31460627: Establishment of BC population and genetic mapping of Psathyrostachys juncea；and The Scientific Achievements Transformation Project of Inner Mongolia Agricultural University YZGC2017012: Breeding and Improvement of Tetroploid Russian Wildrye New Varieties

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    摘要:

    利用38对SSR引物对来源于不同国家与地区的171份野生和栽培新麦草单株种质进行遗传多样性和群体结构分析。研究结果显示，有效引物在新麦草单株材料间共获得308个位点，多态性位点275个，多态性百分率为89.29%，供试新麦草材料的遗传相似性系数介于0.537~0.899，平均为0.742。群体结构分析、主成分分析与NJ聚类法均将供试材料分为3个类群，且类群间的材料相互渗透形成混合类群，分类结果与遗传相似性系数分析表明171份新麦草单株种质分布较集中；3种分类方法对新麦草材料3个不同类群的划分结果基本一致，但混合类群的划分存在差异，每个类群的新麦草单株材料来源背景不同，未表现出明确的地域性规律。

    Abstract:

    Genetic diversity and population structure analysis of 171 wild and cultivated Psathyrostachys individual germplasms from different countries and regions were conducted by 38 pairs of SSR primers. The result by using the effective primers revealed a total of 308 loci among Psathyrostachys individuals, with 275 polymorphic loci and a polymorphism percentage of 89.29%. The genetic similarity coefficient of the tested materials ranged from 0.537 to 0.899 with an average of 0.742. These accessions were divided into three groups by population structure analysis, principal component analysis and NJ clustering. The genetic similarity coefficient analysis showed that the distribution of Psathyrostachys individuals was more concentrated. The classification using three methods generally revealed three different groups of Psathyrostachys materials, despite that there were slightly different in the divisions of mixed groups. Since these Psathyrostachys individuals in each group showed different on genetic background, no clear correlation between geographic distribution and genetic diversity was observed.

    参考文献
    [1]王比德.新麦草生物学和经济学特性的研究[J].中国草地,1990,5:35-38
    [2]白可喻,王培,韩建国.放牧强度对新麦草人工草地氮素在牧草与土壤中的分配和动态的影响[J].草地学报,1999,7(1):46-53
    [3]王勇,徐春波,石凤翎,云锦凤.蒙农4号新麦草种子产量影响因子研究[J].种子,2014,33(9):87-89
    [4]谷安琳,Larry Holzworth,云锦凤.新麦草在内蒙干旱草原的建植试验[J].中国草地,1994,(5):12-14
    [5]于晓丹,张蕴薇.新麦草研究进展[J].草业与畜牧,2010,(1):5-8,13
    [6]赖勇,王晋民,任龙,朱慧琴,马辉,王金贵,巨霞,李宗仁.大麦SSR标记遗传多样性及连锁不平衡分析[J].核农学报,2016,30(10):1889-1897
    [7]刘永财,孟林,张国芳,毛培春,张德罡.新麦草种质遗传多样性的ISSR分析[J]..华北农学报,2009,24(5):107-112
    [8]Wei J Z,Campbell W.F,Wang R.R.-C.Genetic variability in Russian wildrye (Psathyrostachys juncea) assessed by RAPD[J].Genetic Resources and Crop Evolution,1997,44(2):117-125
    [9]罗兵,孙海燕,徐港明,扬志刚,沈宗根,顾雯雯,高阳,郑佳亮.SSR分子标记研究进展[J].安徽农业科学,2013,41(12):5210-5212,5246
    [10]王娟,杜建材,王照兰,刘佳月,李青丰.36份苜蓿种质材料遗传多样性的SSR分子标记[J].中国草地学报,2016,38(2):20-25
    [11]史晓红,郭平仲.分子标记与小麦群体遗传结构分析[J].首都师范大学学报:自然科学版,2000,21(1):59-65
    [12]Liu Z.-W,Biyashev R. M.,Saghai Maroof M.A.Development of simple sequence repeat DNA markers and their integration into a barley linkage map.[J] Theoretical and Applied Genetics,1996,93:869-876
    [13]Struss á,Plieske D J.The use of microsatellite markers for detection of genetic diversity in barley populations[J].Theoretical and Applied Genetics,1998,97:30
    [14]Lakew B R,Henry J,Eglinton J,Baum M,Ceccarelli S,Grando S.SSR analysis of introgression of drought tolerance from the genome of Hordeum spontaneum into cultivated barley (Hordeum vulgare ssp vulgare)[J].Euphytica,2013,191:231-243
    [15]Ramsay L, Macaulay M.A simple sequence repeat-based linkage map of barley[J].Genetics,2000,156:1997-2005
    [16]Varshney R K,Marcel T C,Ramsay L,Russell J,Roder M S,Stein N,Waugh R,Langridge P,Niks R E,Graner A.A high density barley microsatellite consensus map with 775 SSR loci[J].Theoretical and Applied Genetics,2007,114:1091-1103
    [17]郭培国,刘文杰,李海洋,王直亮,夏岩石,李荣华.一种快速有效检测SSR标记的非变性聚丙烯酰胺凝胶的银染方法[J].广州大学学报:自然科学版,2016,15(4):8-12,49
    [18]Evanno G,Regnaut S,Goudet J.Detecting the number of clusters of individuals using the software STRUCTURE:a simulation study[J].Molecular ecology,2005,14(8):2611-2620
    [19]苏龙,徐志健,乔卫华,郑晓明,梁云涛,丁膺宾,刘莎,齐兰,公婷婷,梁新霞,王君瑞,张丽芳,程云连,兰进好,杨庆文.广西药用野生稻遗传多样性分析及SSR引物数量对遗传多样性结果的影响研究[J].植物遗传资源学报,2017,18(4):603-610
    [20]章秋平,刘威生,刘硕,魏潇,刘宁.杏遗传多样性评价所需SSR等位变异数目估计[J].果树学报,2015,32(2):186-191
    [21]张晨,云岚,白亚利,程鹏飞,赵彦.新麦草种质资源的SSR遗传多样性分析[J].中国草地学报,2017,39(5):24-30
    [22]杭焱,金燕,卢宝荣.濒危植物华山新麦草(Psathyrostachys huashanica)的遗传多样性及其保护[J].复旦学报:自然科学版,2004,43(2):260-266
    [23]张云,龙云星,奎丽梅,徐高峰,涂建,辜琼瑶,李华慧,黄平,卢义宣.我国水稻两用核不育系SSR遗传多样性分析[J].分子植物育种,2017,15(7):2836-2846
    [24]Ellstrand N C,Elam R D.Population Genetic Consequences of Small Population Size: Implications for Plant Conservation[J].Annual Review of Ecology and Systematics,1993,24:217-242
    [25]Gupta P K, Rustgi S, Kulwal P L.Linkage disequilibrium and association studies in higher plants: present status and future prospects[J].Plant molecular biology,2005,57(4):461-485
    [26]魏世平,刘晓芬,杨胜先,吕海燕,牛远,章元明.中国栽培大豆群体结构不同分类方法的比较[J].南京农业大学学报,2011,34(2):13-17
    [27]苏一钧,王娇,戴习彬,唐君,赵冬兰,张安,周志林,曹清河.303份甘薯地方种SSR遗传多样性与群体结构分析[J].植物遗传资源学报,2018,19(2):343-351
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张晨,云岚,李珍,等.新麦草种质的SSR遗传多样性及群体结构分析[J].植物遗传资源学报,2019,20(1):48-59.

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  • 收稿日期:2018-06-12
  • 最后修改日期:2018-11-15
  • 录用日期:2018-07-26
  • 在线发布日期: 2019-01-14
  • 出版日期: 2019-01-16
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