In this paper, 44 colored barley germplasms were used as test materials to comprehensively evaluate the multi-index salt tolerance of colored barley germplasm., The germination potential, germination rate, germination index and germination vigor index of the experimental seeds were determined by combining the two color indicators YU (color brightness and concentration) to identify the salt tolerance of the bud stage, and correlation analysis and principal component analysis were used , cluster analysis and other methods to carry out this test. The results showed that the germination rate and germination index were the most sensitive to salt stress response, and germination length and root length are slower to respond to salt stress, and the color brightness and salt tolerance were negatively correlated. The color concentration and salt tolerance were significantly positively correlated. Correlation analysis using F value shows that a positive correlation between the grain color and the salt tolerance, the deeper the color, the stronger the salt tolerance; 44 kinds of colored barley can be divided into 4 categories by comprehensive index F value: (i) four accessions, such as XZDM-1401, XZDM-1404, XZDM-1543, XZDM-1546, which showed strong salt-tolerant; (ii) 16 accessions, such as ZDM-926, XZDM-957, XZDM-972, that showed moderate salt tolerant; (iii) 12 accessions, such as XZDM-815, XZDM-1606 and XZDM-972, that showed weak salt tolerant; (iv) 12 accessions, such as XZDM-973, XZDM-1042 and XZDM-1318, that showed highly salt sensitive.