This study reported the isolation of CiUPF0114 gene from Caragana intermedia, which contained an 876 bp open reading frame, encoding for a protein with 291 amino acids. The predicted isoelectric point and molecular weight of CiUPF0114 were 9.83 and 32.13 kD, respectively. The expression level of CiUPF0114 gene was significantly up-regulated under drought, dehydration or cold treatments. Furthermore by genome-wide screening, a total of 29 UPF0114 gene family members were identified from eight legumes, Arabidopsis and rice. The UPF0114 genes from were divided into subfamily A and subfamily B according to phylogenetic analysis. The A subfamily was further divided into two groups, A1 and A2. CiUPF0114, a gene from Caragana intermedia, belongs to the B subfamily. These results might lay the foundation for further study on the functions of CiUPF0114.