NAC transcription factors are involved in plant abiotic stress response and play important roles for improving plant drought and salt tolerance. Taking advantage of established agrobacterium-mediated transformation platform using the potato cultivar "Atlantic", we generated the transgenic potatoes by expressing Haloxylon ammodendron HaNAC1, and conducted tests for drought resistance with PEG-6000 simulated drought treatment. By analyzing the contents of endogenous hormones, these results showed significant increased content of auxin (IAA, IBA), cytokinin (IP, cZ), jasmonic acid (MEJA, JA-ILE), salicylic acid (MESA), gibberellin (GA3) in three transgenic potato lines if compare to the receptor line (p<0.01, p<0.05). The content of abscisic acid (ABA) was significantly lower than the receptor line. By analyzing the transcriptional level using quantitative PCR analysis, several stress-tolerant genes including NCED1, ERD11, RD29A, DREB2A, LEA3 and KIN1 showed the modified expression in HaNAC1 overexpressing plants to the receptors (p<0.01, p<0.05). Taken together, our results suggested that increased drought resistance by expressing HaNAC1 is associated with the different expression of stress-related genes and hormone content, thus providing a theoretical basis for further molecular mechanism analysis of HaNAC1 response to drought stress.