Soybean mosaic virus SC3 is main prevalent strain of Mosaic virus in huang-huai river and the middle and lower reaches of Yangtze river that causes the yield loss of soybean (Glycine max. ).The resistance of 217 soybean germplasms to SC3 were studied by the method of indoor inoculation identification and by investigating the influence of SC3 on the agronomic and quality traits of different disease-resistant varieties after field inoculation with SC3.It provided data support for the production and application of soybean varieties resistant to Mosaic virus disease and resistant parents for breeding resistant varieties.Virus resistance of 217 soybean germplasm were identified by using artificial inoculation SC3 method in the greenhouse during 2012-2014 years.According to the analysis results, 30 materials (10 disease-resistant varieties, 10 moderately susceptible varieties, and 10 highly susceptible varieties) of different disease resistance varieties were randomly selected. The effects of SC3 on yield - related and quality traits of different varieties were studied by the field inoculation identification in 2013-2014 years on the field..The identification results of 217 soybean germplasms SC3 showed that the disease-resistant varieties of the first class, include 4 symptomless varieties, accounted for 1.84%. Two varieties with high resistance, accounting for 0.92%; 35 varieties with moderate resistance, accounting for 16.13%. In the second class, 92 varieties were moerate susceptible, accounting for 42.40%. The third type of highly susceptible varieties, including 3 susceptible varieties, accounted for 1.38%; 81 varieties were highly susceptible, accounting for 37.33%. SC3 had different effects on the seed weight per plant, plant height, number of nodes on main stem, protein content and oil content of different resistant varieties. The seed weight, plant height, number of nodes on main stem, protein content and oil content of the resistant varieties in the first group were not significantly decreased. The seed weight per plant, number of nodes on main stem, protein content and oil content of the susceptible varieties in the second class did not decrease significantly, and the plant height of the susceptible varieties decreased to different degrees due to the differences of varieties. The seed weight per plant, plant height, number of nodes on main stem, protein content and oil content of the third type of highly susceptible varieties decreased significantly.The studies have shown that SC3 has different effects on the agronomic and quality traits of different resistant varieties, as well as on the agronomic and quality traits of the same disease-resistant varieties. SC3 has no significant influence on most agronomic and quality traits of disease resistant and moderately susceptible varieties, which are suitable for resistance breeding as breeding materials.