Wheat stripe rust, caused by Puccinia striiformis f.sp tritici (Pst), is one of the most destructive disease in winter wheat. This disease seriously threats the production in areas of Gansu province, including Tianshui and Longnan. Planting varieties with genetic resistance was the most economical, environmentally friendly and effective method to control wheat stripe rust. In this article, the recent progress on monitoring virulence of Pst CYR34 and breeding for its resistance in Gansu province was summarized. Took advantage of the practice experiences over the past years, we proposed the future aspects in wheat breeding for CYR34 of Pst. We expect to provide solutions on sustainable management to wheat stripe rust, in order to ensure the security of wheat production in Gansu province of China.