To clarify the distribution status of Pi-ta, Pi-b, Pi54, Pikm genes and their effects on the resistance of rice blast in Huang-Huai-Hai Region, 88 rice varieties collected from this region were detected using the functional markers that specifically target to the four genes. The genetic frequency of Pib+Pi54 was 60.2% within this collection..In parallel, this collection was subjected to test for resistance by natural infection and artificial injection in 2016 and 2017. 87.5% and 77.3% of varieties were susceptible in two years, respectively. This result suggested that the effects of resistant genes were declined. Four genes or gene combinations (Pi-ta, Pi-ta+Pi-b, Pi-ta+Pi-b+Pi54 and Pi-ta+Pi-b+Pi54+Pikm) are applicable in the rice-breeding, due to the resistance to rice blast but with low distribution in the tested varieties. Taken together, the results raised a positive strategy for breeding rice cultivars with rice blast-resistance.