2025年5月10日 7:00 星期六
首页 > 过刊浏览>2019年第20卷第6期 >1606-1612. DOI:10.13430/j.cnki.jpgr.20190225001
PDF HTML阅读 XML下载 导出引用 引用提醒
黄瓜核心种质幼苗耐低温性评价及GWAS分析
作者:
作者单位:

中国农业科学院蔬菜花卉研究所

基金项目:

国家现代农业产业技术体系专项资金（CARS-25）; 中国农业科学院科技创新工程项目（CAAS-ASTIP-IVFCAAS）;中央级公益性科研院所基本科 研业务费专项（Y2017PT52）； 农业部园艺作物生物学与种质创制重点实验室资助项目


Evaluation and Genome-wide Association Study (GWAS) of Low-temperature Tolerance at Seedling Stage in Cucumber Core Germplasm
Author:
Affiliation:

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081

Fund Project:

The Earmarked Fund for Modern Agro-industry Technology Research System (CARS-25);Science and Technology Innovation Program of the Chinese Academy of Agricultural Science (CAAS-ASTIP-IVFCAAS);Central Public-interest Scientific Institution Basal Research Fund(Y2017PT52);Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, P.R.China

  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献
    • |
  • 相似文献
    • |
  • 引证文献
    • | |
  • 文章评论
    摘要:

    本研究以黄瓜核心种质为材料开展苗期耐低温鉴定和材料筛选，并进行全基因组关联分析，挖掘候选基因。在苗期（两叶一心时期），分两批播种，进行自然低温胁迫，两次处理平均温度分别为12℃和19.3℃，分别处理14 d和11 d，根据子叶和真叶的黄化症状进行分级和分组。处理后幼苗差异显著，两批次苗期调查变异系数分别为23.2%和31.7%。将供试核心种质划分为4个组，从87份核心种质材料中筛选出苗期耐低温材料CG45、CG61、CG88和CG104等。对苗期的低温鉴定数据，利用核心种质重测序信息进行GWAS分析，在Chr.1、Chr.3、Chr.4和Chr.5上分别检测到苗期耐低温位点gLTS1.1、gLTS3.1、gLTS4.1和gLTS5.1。其中，位点gLTS5.1对低温敏感，可以被重复检测到。本研究结果对耐低温黄瓜种质的选育及耐低温后续基因挖掘及功能验证具有一定的参考意义。

    Abstract:

    In this study, the core collection of cucumber germplasm accessions were used to evaluate low-temperature tolerance at seedling stage, and the candidate genes were identified via genome-wide association analysis. Two-mature-leaf seedling plants were exposed under low temperature conditions (12℃ or 19.3℃) for 14 days and 11 days, respectively. The classification of low-temperature tolerance was conducted based on the chilling injury index (CII) of cotyledons and true leaves. After treatments, the difference of seedlings was significant, and the coefficient of variation under two environmental conditions were 23.2% and 31.7%, respectively. These accessions were divided into four groups according to CII. Four accessions (CG45, CG61, CG88 and CG104) were shown to be tolerant under low-temperature stress. The genome-wide association mapping of low temperature tolerance identified four loci including gLTS1.1, gLTS3.1, gLTS4.1 and gLTS5.1 that were assigned to the chromosomes 1, 3, 4 and 5 respectively. Among them, the locus gLTS5.1 can be detected repeatedly. Thus, this work provided insight for identifying low-temperature tolerant cucumber germplasm and unlocking the functional genes in future.

    参考文献
    相似文献
    引证文献
引用本文

王伟平,宋子超,薄凯亮,等.黄瓜核心种质幼苗耐低温性评价及GWAS分析[J].植物遗传资源学报,2019,20(6):1606-1612.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2019-02-25
  • 最后修改日期:2019-08-23
  • 录用日期:2019-04-15
  • 在线发布日期: 2019-11-19
文章二维码
您是第位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2025 版权所有
技术支持：北京勤云科技发展有限公司
请使用 Firefox、Chrome、IE10、IE11、360极速模式、搜狗极速模式、QQ极速模式等浏览器，其他浏览器不建议使用!